Synthetically controlling dendrimer flexibility improves delivery of large plasmid DNA
نویسندگان
چکیده
منابع مشابه
Synthetically controlling dendrimer flexibility improves delivery of large plasmid DNA.
Tools for editing the genome and epigenome have revolutionised the field of molecular biology and represent a new frontier in targeted therapeutic intervention. Although efficiencies and specificities of genome editing technologies have improved with the development of TALEs and CRISPR platforms, intracellular delivery of these larger constructs still remains a challenge using existing delivery...
متن کاملSynthetically controlling dendrimer flexibility improves delivery of large plasmid DNA† †Electronic supplementary information (ESI) available: Detailed methods, chemical and cell-based characterization. See DOI: 10.1039/c7sc00097a Click here for additional data file.
School of Molecular Sciences, The Universi Crawley, WA 6009, Australia. E-mail: marck uwa.edu.au Harry Perkins Institute of Medical Resea Australia. E-mail: [email protected] Research School of Chemistry, Australian N Australia ARC Centre of Excellence in Plant Energy Bio 35 Stirling Hwy, Crawley, WA 6009, Austral School of Anatomy, Physiology and Hum Australia, 35 Stirling Hwy, Crawley,...
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UNLABELLED The chlamydiae are important human pathogens. Lack of a genetic manipulation system has impeded understanding of the molecular bases of virulence for these bacteria. We developed a dendrimer-enabled system for transformation of chlamydiae and used it to characterize the effects of inserting the C. trachomatis plasmid into C. pneumoniae, which lacks any plasmids. The plasmid was clone...
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The purpose of this study was to develop a ternary complex of plasmid DNA (pDNA) electrostatically assembled with polyamidoamine (PAMAM) dendrimer and chondroitin sulfate (CS) for effective and secure gene delivery. PAMAM dendrimers are new cationic polymers that are expected to be used as gene delivery vectors. However, cationic non-viral gene vectors showed cytotoxicity by binding to negative...
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ژورنال
عنوان ژورنال: Chemical Science
سال: 2017
ISSN: 2041-6520,2041-6539
DOI: 10.1039/c7sc00097a